DNA Lab
"Quando finalmente interpretati, i messaggi genetici codificati all'interno delle nostre molecole di DNA forniranno le risposte finali alle basi chimiche dell'esistenza umana" James D. Watson.
DNA Lab Facility, with its Sequencing and Nucleic Acid Synthesis units, provides multiple tools for "reading" these messages using automated DNA sequencing using the Sanger method, as well as "writing" new ones using oligonucleotide synthesis.
EQUIPMENT
- Model 3730 DNA Analyzer sequencer (Applied Biosystems)
- Model SeqStudio 24 Flex sequencer (Applied Biosystems)
- Model Biomek i5 robotic station (Beckman Coulter)
- Model OLIGOMAKER nucleic acid synthesizer (OligoMaker Copenhagen)
- Model DROLIGO 48 nucleic acid synthesizer (Biolytic)
- Model ProFlex PCR System, two (Applied Biosystems)
- Model Veriti PCR System, two (Applied Biosystems)
- Model GeneAmp PCR Systems 9700 PCR System, two (Applied Biosystems)
- Model 2720 Thermal Cycler PCR System, two (Applied Biosystems)
SERVICES
Standard Sequencing in Tubes (Plasmids and PCR)
-Premix Tube: a mix of DNA and custom primers under appropriate conditions is contained in the tube.
-Tube: only the DNA that will be purified and sequenced with the Facility's standard primers is contained in the tube.
Standard Sequencing in Plates (Plasmids and PCR)
-Premix Plate: in each well of the plate, organized by column, is a mix of DNA and custom primers under appropriate conditions.
-Plate: in each well of the plate, organized by column, is only the DNA that will be purified and sequenced with the Facility's standard primers.
OLIGONUCLEOTIDE SYNTHESIS
Synthesis of crude and desalted DNA 40 nmol-200 nmol-1 μmol-10 μmol synthesis scales.
Synthesis of crude and desalted DNA with various modifications, including those that can be coordinated, 40 nmol-200 nmol-1 μmol-10 μmol synthesis scales.
Purification of the oligonucleotide by HPLC.
Purification of the oligonucleotide by PAGE (maximum oligo length 120 bases).
