Proteomica e proteomica clinica
Associated Professor of Biochemistry
Supervisor CEINGE Cryopreservation Facility and Proteomics Facility
- 1997: Degree in Chemistry at University Federico II of Naples.
- 1998: Post-graduate fellow at University Federico II of Naples.
- 1999 - 2000: Research Assistant of the Parco Scientifico e Tecnologico dell’Area Metropolitana di Napoli, section of Medical Biotechnologies at CEINGE Advanced Biotechnologies.
- 2000-2003: PhD in Biochemistry and Molecular Biology at Department of Organic Chemistry and Biochemistry, University Federico II of Napoli.
- 2003: Visiting Scientist at Department of Biomolecular Mass Spectrometry, Utrecht University, The Netherlands.
- 2004-2006: Post-doc Researcher at CEINGE Advanced Biotechnologies.
- 2006-2015: Researcher at the Department of Organic Chemistry and Biochemistry, University Federico II of Napoli.
- October 2015-to date: Associate Professor of Biochemistry at the Department of Chemical Sciences at Federico II University of Napoli. Professor of Chemical Biology, Advanced Biochemistry, Structural and Functional Proteomics, Omics Sciences at Department of Chemical Sciences at Federico II University of Napoli.
- July 2019 to date: Head of Cryopreservation Facility at CEINGE Advanced Biotechnologies.
- January 2022-to date: member of the Board of Italian Proteomics Association (ItPA).
- June 2022 to date: Head of Proteomic Facility at CEINGE Advanced Biotechnologies
The scientific activity of Prof. Maria Monti has always been addressed to the elucidation of structure-function relationships in proteins and protein complexes, by using biochemical and proteomics procedures in combination with advanced biomolecular mass spectrometric methodologies applied to biomedical topics.
The main applications are:
- Investigation of protein-protein and protein-ligand interactions by combining biophysics and biochemical approaches, relied on native ES-MS, SPR, cross-linking, selective chemical modifications and limited proteolysis experiments coupled with advanced mass spectrometric methodologies (LiP-MS), proteomics for the characterization of protein interactomes in ex vivo systems, identification and characterization of drugs-targets including binding sites, definition of kinetics and thermodynamics binding parameters;
- Elucidation of the molecular mechanisms of complex biological processes both in physiological and pathological conditions by using functional and differential proteomics approaches. The biological functions exerted in vivo by a specific protein are investigated by the identification of its protein partners (interactome investigation), as well as the alteration in proteome profiles of samples differently treated are unraveled by differential proteomics approaches.
- Characterization of protein post-translational modifications, including assignment of S-S bridges, identification of glycosylation and S-glutathionylation sites, histones epigenetics modifications.
- Viral infections diseases
- Genetics rare diseases
- Neurodegenerative diseases
- Cancer diseases
Flora Cozzolino, Temporary Researcher (RTD-B)
Ilaria Iacobucci, Post-doc
Simona Celentano, Service activity
Irene Cipollone, PhD student
Maria Claudia Gatto, PhD student
Antonella Alfieri, Contractor
Maria Rosaria Mentino, Contractor
D'Angelo G, Uemura T, Chuang CC, Polishchuk E, Santoro M, Ohvo-Rekilä H, Sato T, Di Tullio G, Varriale A, D'Auria S, Daniele T, Capuani F, Johannes L, Mattjus P, Monti M, Pucci P, Williams RL, Burke JE, Platt FM, Harada A, De Matteis MA. Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi. Nature 2013, 501(7465):116-20. DOI: 10.1038/nature12423.
Iacobucci I, Monaco V, Canè L, Bibbò F, Cioffi V, Cozzolino F, Guarino A, Zollo M, Monti M. Spike S1 domain interactome in non-pulmonary systems: A role beyond the receptor recognition. Front Mol Biosci. 2022 Sep 26;9:975570. DOI: 10.3389/fmolb.2022.975570. ISSN: 2296889X
Andolfo I*, Monaco V, Cozzolino F, Eleni Rosato B, Marra R, Cerbone V, Pinto VM, Forni GL, Unal S, Iolascon A, Monti M*, Russo R. Proteome alterations in erythrocytes with PIEZO1 gain-of-function mutations. Blood Advances, 2023, Jun 27;7(12):2681-2693. DOI: 10.1182/bloodadvances.2022008673.
Monaco V., Iacobucci I., Canè L., Cipollone I., Ferrucci V., de Antonellis P., Quaranta M., Pascarella S., Zollo M., Monti M*. SARS-CoV-2 uses Spike glycoprotein to control the host's anaerobic metabolism by inhibiting LDHB Int J Biol Macromol. 2024; 278(Pt 3):134638. doi: 10.1016/j.ijbiomac.2024.134638.
Federti E, Mattoscio D, Recchiuti A, Matte A, Monti M, Cozzolino F, Iezzi M, Ceci M, Ghigo A, Tolosano E, Siciliano A, Ceolan J, Riccardi V, Gremese E, Brugnara C, De Franceschi L. 17(R)-Resolvin D1 protects against sickle cell-related inflammatory cardiomyopathy in humanized mice. Blood. 2025 Apr 24;145(17):1915-1928. doi: 10.1182/blood.2024024768.